{"id":1085,"date":"2017-03-03T12:39:00","date_gmt":"2017-03-03T11:39:00","guid":{"rendered":"http:\/\/www.microgels.rwth-aachen.de\/?p=1085"},"modified":"2020-01-08T14:30:36","modified_gmt":"2020-01-08T13:30:36","slug":"glycan-functionalized-microgels-for-scavenging-and-specific-binding-of-lectins","status":"publish","type":"post","link":"https:\/\/www.microgels.rwth-aachen.de\/?p=1085","title":{"rendered":"Glycan-functionalized microgels for scavenging and specific binding of lectins"},"content":{"rendered":"\n<p>Microgels are promising scaffolds for the presentation of biomolecules such as glycans. Coupling of the cell-surface glycan <em>N<\/em>-Acetyllactosamine (Gal\u03b21,4GlcNAc, LacNAc) to cross-linked polyethylene glycol (PEG) provides a suitable procedure to study multivalent carbohydrate-protein interaction within microgels. By using drop-based microfluidics and radical polymerization, we produced biocompatible monodisperse microgels, which combine star-shaped PEG and allyl-activated LacNAc. The 20 \u00b5m microgels exhibit a wide mesh size and a highly accessible volume as shown by diffusion studies of fluorescent labelled thyroglobulin, a 660 kDa protein. Further, the accessibility of the glycans was investigated with glycan-binding proteins, named lectins. ECL, a galactose binding lectin, shows specific binding to LacNAc-functionalized microgels with a K<sub>d<\/sub> in the micromolar range whereas with GS-II, a GlcNAc specific lectin, no interaction was observed. Concluding, we are able to produce glycan-functionalized microgels which are useful tools to scavenge lectins in biomedical applications.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"http:\/\/www.microgels.rwth-aachen.de\/wp-content\/uploads\/2019\/12\/Jansertal.png\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"500\" src=\"http:\/\/www.microgels.rwth-aachen.de\/wp-content\/uploads\/2019\/12\/Jansertal-1024x500.png\" alt=\"\" class=\"wp-image-1087\" srcset=\"https:\/\/www.microgels.rwth-aachen.de\/wp-content\/uploads\/2019\/12\/Jansertal-1024x500.png 1024w, https:\/\/www.microgels.rwth-aachen.de\/wp-content\/uploads\/2019\/12\/Jansertal-300x146.png 300w, https:\/\/www.microgels.rwth-aachen.de\/wp-content\/uploads\/2019\/12\/Jansertal-768x375.png 768w, https:\/\/www.microgels.rwth-aachen.de\/wp-content\/uploads\/2019\/12\/Jansertal.png 1454w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n\n\n\n<p><em>Alexander Jans, Ruben R. Rosencrantz, Ana D. Mandic\u0301, Naveed Anwar, Sarah Boesveld, Christian Trautwein, Martin Moeller, Gernot Sellge, Lothar Elling, and Alexander J. C. Kuehne<\/em><\/p>\n\n\n\n<p><em>Biomacromolecules <\/em><strong><em>2017,<\/em><\/strong><em> 18 (5), 1460-1465<\/em>, doi: <a href=\"http:\/\/dx.doi.org\/10.1021\/acs.biomac.6b01754\">10.1021\/acs.biomac.6b01754<\/a> <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Microgels are promising scaffolds for the presentation of biomolecules such as glycans. Coupling of the cell-surface glycan N-Acetyllactosamine (Gal\u03b21,4GlcNAc, LacNAc)<\/p>\n","protected":false},"author":1,"featured_media":1087,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"advgb_blocks_editor_width":"","advgb_blocks_columns_visual_guide":"","footnotes":""},"categories":[34],"tags":[],"class_list":["post-1085","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-highlights"],"author_meta":{"display_name":"sfb985_admin","author_link":"https:\/\/www.microgels.rwth-aachen.de\/?author=1"},"featured_img":"https:\/\/www.microgels.rwth-aachen.de\/wp-content\/uploads\/2019\/12\/Jansertal-300x146.png","coauthors":[],"tax_additional":{"categories":{"linked":["<a href=\"https:\/\/www.microgels.rwth-aachen.de\/?cat=34\" class=\"advgb-post-tax-term\">Highlights<\/a>"],"unlinked":["<span class=\"advgb-post-tax-term\">Highlights<\/span>"]}},"comment_count":"0","relative_dates":{"created":"Posted 9 years ago","modified":"Updated 7 years ago"},"absolute_dates":{"created":"Posted on March 3, 2017","modified":"Updated on January 8, 2020"},"absolute_dates_time":{"created":"Posted on March 3, 2017 12:39","modified":"Updated on January 8, 2020 14:30"},"featured_img_caption":"","series_order":"","_links":{"self":[{"href":"https:\/\/www.microgels.rwth-aachen.de\/index.php?rest_route=\/wp\/v2\/posts\/1085","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.microgels.rwth-aachen.de\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.microgels.rwth-aachen.de\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.microgels.rwth-aachen.de\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.microgels.rwth-aachen.de\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1085"}],"version-history":[{"count":2,"href":"https:\/\/www.microgels.rwth-aachen.de\/index.php?rest_route=\/wp\/v2\/posts\/1085\/revisions"}],"predecessor-version":[{"id":1094,"href":"https:\/\/www.microgels.rwth-aachen.de\/index.php?rest_route=\/wp\/v2\/posts\/1085\/revisions\/1094"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.microgels.rwth-aachen.de\/index.php?rest_route=\/wp\/v2\/media\/1087"}],"wp:attachment":[{"href":"https:\/\/www.microgels.rwth-aachen.de\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1085"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.microgels.rwth-aachen.de\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1085"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.microgels.rwth-aachen.de\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1085"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}